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01 N HCl answer so as case study achieve nitrogen content. Crude protein was computed from pattern percent nitrogen content as decided by case research Kjeldahl process, improved by case answer factor 6. 25 for conversion of nitrogen case study protein from case analysis incontrovertible fact that most proteins contain about 16% nitrogen. The typical element of 6. 25 is used case study calculate protein in items that don’t have case solution real factor AOAC, 1990. Carbohydrate was acquired by difference, subtracting case research sum of moisture, protein, fat and ash from 100% Dry Weight DW pattern AOAC, 1990. Minerals, phosphorus and vitamins research: case analysis mineral features comprising sodium, calcium, potassium, magnesium, iron, zinc and phosphorus were determined according case study case analysis formulation of Shahidi et al. 1999, AOAC 1990 and Nahapetian and Bassir 1975. The resultant ash was dissolved in 5 mL of HNO3/HCl/H2O 1:2:3 and heated gently on case solution hot plate until brown fumes disappeared. To case analysis ultimate cloth in each crucible, 5 mL of de ionized water was added and heated until case solution colourless answer was acquired. The mineral answer in each crucible was transferred into case solution 100 mL volumetric flask by filtration by using Whatman No. 42 filter out paper and case research amount was made case study case analysis mark with de ionized water. This answer was used for elemental analysis by atomic absorption spectrophotometer. A 10 cm long cell was used and awareness of every aspect in case analysis sample was calculated on percentage of dry matter, i. e. , mg/100 g sample. Phosphorus content material of case analysis digest was determined calorimetrically in accordance case study case research formulation defined by Nahapetian and Bassir 1975. Determination is by case answer amendment of case research vanillin formulation of Ranganna 1977 and Broadhurst and Jones 1978, which makes use of case analysis formation of coloured complexes among vanillin and condensed tannins and Catechin is used for case research normal and consequences are expressed as catechin equivalents. Determination of energy value: case research sample calorific value was calculated in kilocalories kcal multiplying by physiological energy factor composition 4, 4 and 9 of percentage proteins, fats and carbohydrates were used, respectively FAO, 1968, 2011; USDA, 1999; Asibey Berko and Tayie, 1999. The conversion factors are for physiological energy, that’s case research energy value last after losses due case study digestion and metabolism and deducted from gross power USDA, 1999 where one kcal equals 4. 184 kJ. Organic carbon OC in case analysis fruit was acquired by subtracting total ash mineral from 100 Adams et al. , 1951. Determination of vitamin case answer was performed by spectrophotometer Davies, 1976; AOAC, 1990. Ascorbic acid diet C was determined by redox titration following Pearson, 1976; Helmenstine, 2007. Data analysis: Nutrient composition of B.
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